100 mm l-ascorbic acid solution Search Results


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European Directorate for the Quality of Medicines and HealthCare acetylsalicylic acid asa
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Selleck Chemicals osteogenic media
Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM <t>osteogenic</t> medium.
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Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM <t>osteogenic</t> medium.
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Cell Signaling Technology Inc osteogenic medium
Figure 2: PDGF-BB enhanced BMSC <t>osteogenic</t> differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.
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FUJIFILM l -ascorbic acid
Figure 2: PDGF-BB enhanced BMSC <t>osteogenic</t> differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.
L Ascorbic Acid, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PChem Associates Inc vitamin c (l-ascorbic acid) nd nd pchem 0005 hplc (mg/100 g)
Figure 2: PDGF-BB enhanced BMSC <t>osteogenic</t> differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.
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Merck KGaA l-ascorbic acid aa
Figure 2: PDGF-BB enhanced BMSC <t>osteogenic</t> differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.
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Thermo Fisher low glucose dulbecco s modified eagle s medium
Figure 2: PDGF-BB enhanced BMSC <t>osteogenic</t> differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.
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Image Search Results


Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM osteogenic medium.

Journal: Frontiers in Physiology

Article Title: Thiamet-G facilitates reparative dentin formation via modulating O-GlcNAcylation and inflammation

doi: 10.3389/fphys.2025.1739168

Figure Lengend Snippet: Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM osteogenic medium.

Article Snippet: After 24 h, the media was changed with osteogenic media (Alfa-MEM with 1% penicillin-streptomycin, 5% fetal bovine serum, 50 μM L-ascorbic acid, 10 mM β-glycerophosphate, and 100 nM dexamethasone) in presence or absence of Thiamet-G (S7213, Selleckchem.com ) with various concentrations.

Techniques: MTS Assay, ALP Activity Assay, Activity Assay, Control, Expressing, Negative Control

Figure 2: PDGF-BB enhanced BMSC osteogenic differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.

Journal: Stem cells international

Article Title: The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration.

doi: 10.1155/2018/3272098

Figure Lengend Snippet: Figure 2: PDGF-BB enhanced BMSC osteogenic differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.

Article Snippet: For osteogenic differentiation, cells were seeded in 12-well plates and cultured in osteogenic medium (DMEM, 10% FBS, 1% penicillin/ streptomycin, 50μg/mL L-ascorbic acid, 10mM glycerophosphate, and 100 nM dexamethasone) with or without PD98059 (10μM), an extracellular signal-related kinase (ERK) inhibitor (Cell Signaling Technology, Inc.).

Techniques: Protein-Protein interactions, Expressing, Staining, Activity Assay